DESCRIPTION:

Human recombinant BPTF PHD-bromodomain construct. Full-length BPTF1 is a DNA and histone-binding component of the NURF nucleosome remodeling complex. This construct comprises BPTF’s second PHD zinc-finger, which primarily binds histone H3K4me3, and the adjacent bromodomain, which has binding affinity for various histone tail acetyllysines, including H4 K5Ac, K12Ac and K16Ac. BPTF expression is elevated in developing neurons, but also in neuronal tissue under various neurodegenerative conditions. Amplification of the BPTF-coding chromosomal locus is prevalent in various cancers and knockdown of BPTF can restrict proliferation in cultured cells with an engineered pre-malignant phenotype.

ACCESSION #: NM_182641

Uniprot Link

INCLUDES AMINO ACIDS: 2722-2920 (C-term.)

Tag: N-terminal His-tag

MW: 26.0 kDa

EXPRESSION SYSTEM: E. coli

SUPPLIED AS: Solution of purified recombinant protein in 50 mM Tris-HCl, pH 7.5, 500 mM NaCl, 1 mM TCEP, 10% glycerol (v/v)

STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted protein is not recommended.

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Substantial Discounts Available on Bulk Quantities

We also offer assay services for BPTF-[PHD-BRD] (His).